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Use: Fill in the amount of cDNA (ng), dNTPs (ng), primers/probe (ng), the efficiency of the primer-probe, the quality of the RNA (RIN-number), the name of the sample and the number of cycles. Press 'Add Sample' to add it to the list. After adding all the samples press 'Calculate' to start the reaction. An image of the amplification plot can be saved in the directory of the program and the results can be seen in the command window by clicking...